A assembled plate column bioreactor for adherent cell culture

Abstract

The ideal bioreactor should be highly efficient, cost-effective and able to promote cells adhesion growth. In this study, we constructed a plate-tower bioreactor with S-loop in each layer based on multilayer plate bioreactor and plate column theory. Human hepatocellular carcinomas(HepG2) and human normal liver cell L02 (L02) cells were employed evaluation of bioreactor performance. The results show that cells were able to adhere to the plates and the density reached to 1～1.2 × 105/cm2. When circulating flow rate of the reactor is 8 ml/min and the oxygen supply concentration is 40 %, the sufficient material and gas exchange in bioreactor, the amount of albumin, urea and cytochrome P450(CYP450) content in the hyperoxia group were 3.31, 2.58 and 2.6 times higher than that of the control group, there was no obvious cell damage. This condition up the regulation of genes in more metabolic pathways and improved the metabolic capacity of hepatocytes. The single-layer area and the number of bioreactors can be flexibly adjusted, and the manufacturing cost of single-layer is close to that of ordinary plastic cell culture dishes. Either batch or continuous mode can be used in the bioreactor. In view of the above characteristics, the bioreactor is more conducive to practical application.