Abstract
Summary Proteins specifically bound to DNA have been implicated to modulate transcription. For the purpose of identifying proteins bound to Tetrahymena rDNA, extrachromosomal nucleoli were isolated and their proteins analyzed. A 34 kDa protein was found to be strongly bound to actively transcribing rDNA, the binding being resistant to 0.5 % Sarkosyl. Fractionation of restriction enzyme digested rDNA chromatin fragments suggested the binding is enriched in a central spacer and/or transcribed gene region of rDNA.
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