Abstract
In the present study, thirty autosomal insertion and deletion polymorphic loci were simultaneously amplified and genotyped in a multiplex system, and their allelic frequencies as well as several forensic parameters were obtained in a sample of 236 unrelated healthy Tujia individuals. All the loci were in Hardy-Weinberg equilibrium after applying a Bonferroni correction and all pair-wise loci showed no significant linkage disequilibrium. These loci were observed to be relatively informative and discriminating, quite efficient for forensic applications. Allelic frequencies of 30 loci were compared between the Tujia group and other reference populations, and the results of analysis of molecular variance indicated the Tujia group showed the least significant differences with the Shanghai Han at one locus, and the most with Central Spanish population at 22 loci. We analyzed the population genetic structure by the principal component analysis, the clustering of STRUCTURE program and a Neighbor-Joining tree, and then evaluated the genetic relationships among Tujia and other 15 populations.
Highlights
Short tandem repeats (STRs) have become popular DNA markers in forensic DNA labs for more than 20 years and have been proved to possess several benefits, which make them especially suitable to identify victims, perpetrators, missing persons, and for kinship testing and population genetic analysis[1,2,3,4,5]
We firstly reported the population genetic data of 30 insertion and deletion polymorphisms (InDels) in Chinese Tujia ethnic group, evaluated their usefulness in the field of forensic sciences, analyzed the interpopulation differentiations, and retraced the genetic background of the Tujia group by the population structure construction, principal component analysis, phylogenetic tree and some other analyses
The genotype frequency data for all loci showed no deviations from Hardy-Weinberg equilibrium (HWE) expectations in the sample of Tujia group
Summary
Short tandem repeats (STRs) have become popular DNA markers in forensic DNA labs for more than 20 years and have been proved to possess several benefits, which make them especially suitable to identify victims, perpetrators, missing persons, and for kinship testing and population genetic analysis[1,2,3,4,5]. The AMOVA comparison results showed significant differences between the Tujia group and Shanghai Han, Beijing Han, Guangdong Han, She, Xibe, South Korean, Tibetan, Yi, Uigur, Kazak, Uruguayan, Hungarian, Basque, Dane, Central Spanish populations at 1, 3, 3, 4, 5, 7, 8, 9, 14, 14, 20, 20, 20, 21 and 22 loci, respectively.
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