Abstract
We have isolated a full-length murine clone corresponding to the rat neuronal p1A75 partial cDNA (Sutcliffe, J. G., Milner, R. J., Shinnick, T. M., and Bloom, F. E. (1983) Cell 33, 671-682). It encodes a 185-residue polypeptide that displays 56% identity with p19, a protein selectively expressed in the Golgi apparatus of neural cells (Sabéran-Djoneidi, D., Marey-Semper, I., Picart, R., Studler, J.-M., Tougard, C., Glowinski, J., and Lévi-Strauss, M. (1995) J. Biol. Chem. 270, 1888-1893). An antibody directed against the recombinant polypeptide allowed us to demonstrate the existence of the natural 21-kDa protein (p21) in brain and its prominent juxtanuclear Golgi-like localization in cultured neurons. Ultrastructural observation of cultured neurons and analysis of transfected COS cells revealed a specific labeling of the Golgi apparatus, suggesting, as for p19, the presence of a Golgi targeting signal in its primary sequence. Surprisingly, p21, which is much more strongly expressed in the olfactory epithelium than p19, is also present in the Golgi complex of spermatocytes and in the flagellar middle piece of late spermatids.
Highlights
We have previously described a 19-kDa murine protein (p19) selectively expressed in the Golgi apparatus of neural and neuroendocrine cells whose human corresponding gene has been localized in 5q35 [1, 2]
Searches in protein data bases for other members of this new family have only revealed that these two proteins share a highly similar short segment with secretogranin III, which is expressed in intracellular vesicles of neural cells [1, 5]
Unlike p19, which is absent from the testis and faintly expressed in the olfactory epithelium, p21 is very strongly expressed in the olfactory epithelium and in male germ cells
Summary
We have previously described a 19-kDa murine protein (p19) selectively expressed in the Golgi apparatus of neural and neuroendocrine cells whose human corresponding gene has been localized in 5q35 [1, 2]. The primary sequence of p19 showed a 57% similarity with the translation product of an open reading frame of the rat neuronal p1A75 [3] partial cDNA, which was isolated 14 years ago and whose human corresponding gene is localized in 4p16 [4].
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