Year-end Sale % OFF 
Abstract
Introduction Clonorchis sinensis is a major parasite affecting the Korea population. Despite the high infection rate and pathogenicity, very few studies have been conducted to investigate the immune responses against the proteins of C. sinensis.MethodsIn this study, in vitro immune response induced by a recombinant 21.6 kDa tegumental protein derived from C. sinensis (rCsTegu21.6) was confirmed in murine dendritic cells and T cells. For the in vivo analysis, each mouse was immunized three times. Total serum IgG and T cell cytokine production were determined by ELISA, while T cell proliferation was detected by a WST (Water‐Soluble Tetrazolium salt)‐1 assay.ResultsIn vitro tests indicated that rCsTegu21.6 treatment increased the expression of surface molecules, such as CD40 (77%), CD80 (52%) and CD86 (46%), on murine dendritic cells and the secretion of cytokines (TNF‐α, IL‐6, IL‐1β, IL‐10, and IL‐12p70). Moreover, co‐culturing dendritic cells activated by rCsTegu21.6 with allogenic T cells induced T cell proliferation over time. rCsTegu21.6 also stimulated specific antibody production and cytokine secretion [IL‐2, IL‐4, and interferon (IFN)‐γ)] from T cells following immunization in vivo. Notably, rCsTegu21.6 predominantly induced IgG1 production and secretion of the Th2 cytokine IL‐4, regardless of the type of adjuvant used.ConclusionThese results serve as a foundation for the development of tegumental protein‐based vaccines against C. sinensis.
Highlights
Clonorchis sinensis is a major parasite affecting the Korea population
C. sinensis was classified as a probable carcinogen in 1994 by the International Agency for Research on Cancer (IARC) of the World Health Organization (WHO)
In the immunohistochemical and localization analyses, CsTegu21.6 was mainly located in the tegument of adult C. sinensis (Figure 1C) and was not observed in other tissues
Summary
Clonorchis sinensis is a major parasite affecting the Korea population. Despite the high infection rate and pathogenicity, very few studies have been conducted to investigate the immune responses against the proteins of C. sinensis. Methods: In this study, in vitro immune response induced by a recombinant 21.6 kDa tegumental protein derived from C. sinensis (rCsTegu21.6) was confirmed in murine dendritic cells and T cells. Adult C. sinensis can survive in the bile ducts for an extended period, leading to the development of various diseases, including jaundice, indigestion, biliary inflammation, bile duct obstruction, cholelithiasis, cholestasis, cholangitis, cholecystitis, liver cirrhosis, and most severely, bile duct cancer or cholangiocarcinoma (CCA) [1,2,3]. Because of these risks, C. sinensis was classified as a probable carcinogen (group 2A) in 1994 by the International Agency for Research on Cancer (IARC) of the World Health Organization (WHO). In a 2008 case-control study in Korea, radiological evidence of C. sinensis and recent consumption of raw freshwater fish were significantly correlated with gallstone
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
