Abstract
BackgroundPeroxiredoxins (Prxs) are a large family of antioxidant enzymes that respond to biotic and abiotic stress by decomposing reactive oxygen species (ROS). In this study, the stress tolerance function of the Th2CysPrx gene was further analysed. It lays a foundation for further studies on the salt tolerance molecular mechanism of T. hispida and improved salt tolerance via transgenic plants.ResultsIn this study, the stress tolerance function of the Th2CysPrx gene was further analysed. The results of transgenic tobacco showed higher seed germination rates, root lengths, and fresh weight under salt stress than wild-type tobacco. Simultaneously, physiological indicators of transgenic tobacco and T. hispida showed that Th2CysPrx improved the activities of antioxidant enzymes and enhanced ROS removal ability to decrease cellular damage under salt stress. Moreover, Th2CysPrx improved the expression levels of four antioxidant genes (ThGSTZ1, ThGPX, ThSOD and ThPOD).ConclusionsOverall, these results suggested that Th2CysPrx enhanced the salt tolerance of the transgenic plants. These findings lay a foundation for further studies on the salt tolerance molecular mechanism of T. hispida and improved salt tolerance via transgenic plants.
Highlights
Peroxiredoxins (Prxs) are a large family of antioxidant enzymes that respond to biotic and abiotic stress by decomposing reactive oxygen species (ROS)
The Real-time quantitative PCR (qRT-PCR) results showed that Th2CysPrx gene were overexpressed in all 14 lines (Additional file 1: Figure S1)
The results showed that, under normal conditions, there was no obvious difference in the germination rates and seedling growth between transgenic and WT plants (Fig. 1a-c)
Summary
Peroxiredoxins (Prxs) are a large family of antioxidant enzymes that respond to biotic and abiotic stress by decomposing reactive oxygen species (ROS). TheIF1A, ThDREB, ThZFP1, ThGSTZ1 and ThPOD3 in T. hispida increase salt and drought tolerance by regulating superoxide dismutase (SOD) and peroxidase (POD) activities to reduce reactive oxygen species (ROS) accumulation [7,8,9,10,11]. These results all suggest that scavenging of ROS plays an important role in the response of T. hispida to salt stress
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
