A 1Ns Disomic Addition from Psathyrostachys Huashanica Keng Confers Resistance to Powdery Mildew in Wheat

Jing Han,Jun Wu,Xinhong Chen,Yuxiu Liu,Qunhui Yang,Chenchen Hou,Jinglin Wang,Qiaoying Zhang,Jiachuang Li

doi:10.3390/agronomy10020312

Jing Han, Jun Wu + Show 7 more

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https://doi.org/10.3390/agronomy10020312

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Journal: Agronomy	Publication Date: Feb 24, 2020
Citations: 8	License type: CC BY 4.0

Affiliation: North West Agriculture and Forestry University

Abstract

Powdery mildew is a fungal disease that threatens wheat production throughout the world. Breeding resistant cultivars is an effective way to reduce harm caused by powdery mildew. In this study, 35 wheat-Psathyrostachys huashanica-derived lines were developed by crossing common wheat and P. huashanica Keng (2n = 2x = 14, NsNs) using embryo culture. Resistance to powdery mildew in the derived lines was identified at the seedling and adult stages. Line H5-5-4-2 was selected with immunity to powdery mildew at both growth stages. The chromosome structure of this line was characterized by cytology, genomic in situ hybridization (GISH), and expressed sequence tag-sequence-tagged site (EST-STS) analysis. The chromosome configuration was 2n = 44 = 22II. Two P. huashanica chromosomes with strong hybridization signals were detected by GISH analysis. Among 83 EST-STS markers that covered all seven homologous groups in wheat, three pairs of STS markers, BE497584, BF202643, and BG262410, located in wheat homologous group 1 amplified clear specific bands related to P. huashanica. The results indicated that resistant line H5-5-4-2 was a wheat-P. huashanica 1Ns disomic addition line.

Highlights

Wheat (Triticum aestivum L.) is one of the most widely cultivated cereal crops worldwide and at least one third of the global population depends on wheat as the staple food [1]
The aims of the study were: (a) to evaluate the resistance to powdery mildew in 35 wheat-P. huashanica-derived lines; (b) to determine the chromosomal composition and genomic origin of the resistant materials based on cytogenetic observations and genomic in situ hybridization (GISH)
H5-5-4-2 was selected for further study because of its immunity to powdery mildew

Summary

Introduction

Wheat (Triticum aestivum L.) is one of the most widely cultivated cereal crops worldwide and at least one third of the global population depends on wheat as the staple food [1]. Fungicides are often used to control powdery mildew but their widespread application is inappropriate due to high cost, development of resistance in the pathogen, and environmental impacts [4]. Breeding resistant cultivars is extremely important and this is the main method employed for effectively controlling powdery mildew in wheat [5]. Pathogen populations undergo rapid mutation events and the coevolution with host resistance that cause resistance gene to become ineffective [6,7]. It is necessary to discover more resistance resources that confer resistance to powdery mildew for application in wheat breeding [8]. Distant hybridization is an effective method for broadening the resistance spectrum by introducing novel resistance genes from wild relatives into the bread wheat gene pool [9]

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