Abstract
Abstract Background The detection of NRBCs in the peripheral blood is a marker of many pathological conditions andhas significant prognostic value. Microscopy is the traditional method for NRBC count, but it’s time consuming and has low precision and efficacy and requires experienced technicians. TheMindray BC-6800 Plus is a hematological analyzer that brings SF Cube technology, whichquantifies NRBCs in the WNB channel. This channel differentiates basophils and NRBCs, andcounts white blood cells. The present study aims to evaluate if the automated method is preciseand comparable to the manual microscopy. Methods Blood samples from 40 patients with NRBC flag from the laboratory routine were evaluated bymicroscopy. Blood films were prepared automatically with a Mindray SC-120 slidemaker/stainer.NRBC were manually counted and reported as number of NRBC per 100 white cells, which wasconsidered the reference method. The results of microscopic counts were compared with thoseof the automated method and statistically analyzed using the F-test, T-test and Pearson’ scorrelation coefficient. Results The results show good correlation between the methods: F-test of 1.36 (below 1.69); t test (0,2791) below the critical T(2,0210) and Pearson’s correlation coefficient was 0.9, which isconsidered an adequate result. Samples used in this evaluation comprised mainly low results andfew samples with higher counts. The maximum observed value was 41%, which was confirmedby microscopy. It is important that samples with larger counts be evaluated to be sure of thelinearity value of the test. Conclusion The results demonstrated that the NRBC counts obtained by Mindray BC-6800 analyzer are inagreement with data from manual counts. However, it is necessary that the accuracy of sampleswith very high counts be investigated and compared with the reference method. Thisimplementation of the automatic release of NRBC can allow the laboratory to reduce the time forreleasing tests and optimize the productivity of microscopists.
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