A 15.13 kDaEnterobacter gergoviae Biofilm Predictive Protein is Recognized bysIgA in Bronchial Aspirate from Patients with VAP

Abstract

Ventilator-associated pneumonia (VAP) is difficult to diagnose, expensive to treat, and is associated with significant morbidity and mortality. The major part of VAP pathogenesis is the formation of a biofilm structure which also plays role in its resistance to treatment. A strategy to preventVAP isby removing the biofilm structure. The aim of this study is looking up the biofilm predictive protein of Enterobacter gergoviae (E. gergoviae) which is recognized by Secretory Immunoglobulin A (sIgA) as a mucosal immune response in VAP patient. This study used a tube adherence method for the detection of biofilms, protein profiling techniques with SDS PAGE and blotting for the evaluation of the antigen antibody response. The sIgAwere isolated from bronchial aspirate of VAP patient at ICU RSSA Malang. From two E. gergoviae samples, one sample biofilm positive (sample 1), and theother was biofilm negative (sample 2). The biofilm predictive protein was detected using SDS PAGE, and is discovered to be 15.13 kDa. The sIgA from sample 1 (p 1) reacted with protein band 15.13 kDa, 38.02 kDa, 45.71 kDa and 47.21 kDa whilesIgA from sample 2 (p 2) reacted with 38.02 kDa, 45.71 kDa, 47.21 kDa, and 50 kDa. This finding was predicted that protein MW 15.13 kDa played role in biofilm formation.