Abstract
This paper describes a reserved-phase high performance liquid chromatographic method for detecting melamine (MEL) and related analogues, cyanuric acid (CYA), ammeline (AML), and ammelide (AMD), using a 100% water mobile phase. Chromatographic separation was performed an Inertsil? ODS-4 (250 × 4.6 mm, 5 μm) with a water mobile phase and a photodiode-array detector. The monitoring wavelength was adjusted to 210 nm which represents an average maximum for all the analytes. The total run time was < 8 min. The method shows high stability, significant linearity and satisfactory sensitivity. The detection limits were established in the range 23 - 46 ng.mL–1. An inexpensive and harmless method for the simultaneous detection of MEL, CYA, AML, and AMD was developed and may be further applied to the quantification in foods.
Highlights
In 2007, pet foods adulterated melamine resulted in the serious illness of animals that consumed the food
This paper describes a reserved-phase high performance liquid chromatographic method for detecting melamine (MEL) and related analogues, cyanuric acid (CYA), ammeline (AML), and ammelide (AMD), using a 100% water mobile phase
MEL used for the adulteration contains three related analogues, byproducts in the manufacturing of MEL, namely cyanuric acid (CYA), ammeline (AML), and ammelide (AMD) (Figure 1) [3]
Summary
In 2007, pet foods adulterated melamine resulted in the serious illness of animals that consumed the food. The US Food and Drug Administration issued new methods for the analysis of melamine in liquid formula, based on LC-MS/MS detection [12] These methods have crucial drawbacks: 1) all of the methods consume large quantities of organic solvents in the mobile phases. The facilities that LC-MS/MS system is available are limited to part of industrial nations because these are hugely expensive, and the methodologies use complex and specific These are unavailable in a lot of laboratories for routine analysis, in developing countries. As the first examination problem in the establishment of an international harmonized method for the residue monitoring of MEL, CYA, AML, and AMD, this paper describes a 100% water mobile phase HPLC conditions to detect the four compounds without organic solvent consumption
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