A 1-aminocyclopropane-1-carboxylate deaminase MrACCD from Metarhizium robertsii is associated with plant growth promotion for Metarhizium spp

Abstract

Besides killing insects, Metarhizium spp. have shown another ecological role as plant associates. Partial genra and groups of these entomopathogenic fungi act as plant growth promoters during root colonization. Here, we report that Metarhizium robertsii produces a 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD encoded by MracdS, MrACCD), which is involved in promoting early vegetative growth in wheat, while Metarhizium acridum lacks a functional ACCD, although a gene encoding for a putative ACCD has been identified in its genome. MracdS expression was up-regulated by a max 10.7-fold with 3 mM ACC and high ACCD enzymatic activities were induced by either ACC (7.5-fold) or wheat root (3.2-fold). In contrast, no ACCD activity was detected in M. acridum in the presence of both inducers. In pot assay, wheat seeds were treated with wild-type M. robertsii (Mr23), wild-type M. acridum (Mac324), MracdS disruption mutant (ΔMracdS) and M. acridum transformant harboring heterologous MracdS (Mac324-MracdS). Relative to the control seeds treated with heat-killed conidia, Mr23, ΔMracdS and Mac324-MracdS increased root length (by 66.2, 31.8 and 40.2%), and plant biomass (by 56.6, 42.1 and 40.9%). Nevertheless, ΔMracdS deficient in ACCD activity heavily impaired its capability of wheat growth promotion by decrease of 20.7% in root length relative to Mr23. In addition, Mr23 and Mac324-MracdS also increased shoot growth (by 42.3, and 42.7%) while ΔMracdS failed. Mac324 showed no effect on plant growth during the test. These data suggest a role for ACCD in the plant growth promotion effect by M. robertsii, which is irrelevant to Metarhizium colonization of roots since rhizosphere competency of both Mr23 and Mac324 are unaffected by the change of ACCD activity.