Abstract
Delivery of antibody to immunoglobulin G deficient patients is most efficient when given intravenously. However, Cohn fraction II must be processed further to prevent spontaneous complement activation by IgG aggregates. Multiple lots of three different IgCs prepared for intravenous use (IVIgG) were tested for antibody titers and opsonic activity for Hemophilus influenzae type b (HiB) to assess whether production processes reduced opsonic activity. Anti-HiB IgG titers were measured by an enzyme-linked immunosorbent assay (ELISA) using whole bacteria as antigen. Opsonic activity was measured as the relative peak neutrophil chemiluminescence (CL) elicited by HiB opsonized with each IVIgG tested. All data was normalized to the results of heated serum from a normal adult with high opsonic activity for HiB. None of the differences in HiB titer were statistically significant. However, the peak neutrophil CL achieved varied according to the IVIgG used for opsonization (p<0.05 for all pairs by the Neuman-Keul's t-test). While not altering antibody content, some methods of preparation of IgG to permit intravenous use may decrease opsonic activity.
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