Abstract
Immunogens that induce neutralizing antibodies to HIV-1 are essential components of a preventative vaccine. The HIV-1 Envelope (Env) contains conserved neutralizing epitopes, defined by monoclonal antibodies derived from infected individuals. We hypothesize that directed molecular evolution can create novel Env variants that render these neutralizing epitopes more immunogenic. We used DNA shuffling to generate novel, chimeric Env variants from wild type genes. These included clade B gp120 and gp120 Core as well as consensus clade A, B, C and Group M gp140 sequences. DNA shuffling is an efficient method for extensively recombining parental genes in vitro to create highly functional recombinant libraries.
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