Abstract
A recent investigation revealed that triphenyl tetrazolium chloride (TTC) staining had a significant effect on myocardial uptake determinations of radiolabeled tracers with experimental infarction/reperfusion studies. We therefore investigated the impact of TTC staining on technetium-99m-Q12 and thallium-201 myocardial uptake in canines during infarction. Ten adult male mongrel dogs underwent a left anterior descending occlusion for 2 hours and subsequent injection of Q12 or thallium with 30 minutes of circulation. The whole heart was then excised, washed with saline, and myocardial counts were determined by a well counter. The heart was then perfused with 1% TTC stain for 5–10 minutes and then recounted. (expressed as μCi ± SD) are below: 99m Q12(n = 5) Thallium-201 (n = 5) Pre-TTC 175 ± 57 44 ± 15 post-TTC 173 ± 57 36 ± 11 * % Change 1 ± 1% 16 ± 12% * * p < 0.05 from baseline There is no effect of TTC staining by coronary artery perfusion on the reliability of Q12 myocardial uptake measurement but thallium-201 retention is significantly reduced. TTC staining may have an important impact on the reliability of experimental infarction/reperfusion studies using thallium-201 but not 99m Tc-Q12.
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