#953 Preliminary results of the peripheral T follicular cells and their subsets in ANCA associated glomerulonephritis

Abstract

Abstract Background and Aims T cells play crucial role in the pathogenesis of autoimmune diseases such as ANCA associated vasculitis-glomerulonephritis (AAV/GN) by secreting immune mediators and helping B cell-mediated long-lived humoral immunity development. Specific T and B cells subsets can be used as biomarkers of patients with AAV/GN. We aimed to prospectively compare the predictive ability of such subpopulations in patients with active AAV/GN. Method Flow cytometry was applied in the peripheral blood of 29 subjects. 15 AAV/GN patients (M/F 6/9), age: 62.1(28-82)years, MPO/PR3/Negative ANCA: 10/4/1) and 14 healthy controls (HC), to estimate T-cells subsets: CD4, T-folicular cells (Tfol), T-regulatory cells (Tregs), Tfh, Tfh1, Tfh2, Tfh17, and T-folicular regulatory cells(Tfr) and B-cells subsets: CD19, IgD(+)CD127(-), IgD(+)CD127(+), IgD(-)CD127(+) and IgD(-)CD127(-). Results Compared to HC, AAV/GN patients had reduced CD4 (698.8 Vs 368.8, p = 0.04) but increased proportion of Tfol, and both Tfh1 and Tfh2 cells (7% vs 12%, p = 0.05, 8.4 vs 11.2, p = 0.03, 13.3 vs. 45.9, p = 0.09, respectively). Both Tfr (p = 0.048) and Tfh (p = 0.023) were also significantly higher in group A, compared to group HC. CD19 levels were lower in AAV/GN compared to HC, with reduced all subpopulation, though of not-statistical significance. Conclusion Specific T-cells subsets can be measured in AAV/GN patients’ peripheral blood. Subpopulations such as Tfh and Tfr can be used as biomarkers to diagnose patients with active disease and their role in the pathogenesis of AAV/GN must be studied in detail to determine if they can become targets for new therapies in the future.