950 THE EFFECT OF HONEY ON MAST-CELL DEGRANULATION: A POSSIBLE ROLE IN PAINFUL BLADDER SYNDROME/INTERSTITIAL CYSTITIS (PBS/IC)

Abstract

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Interstitial Cystitis1 Apr 2011950 THE EFFECT OF HONEY ON MAST-CELL DEGRANULATION: A POSSIBLE ROLE IN PAINFUL BLADDER SYNDROME/INTERSTITIAL CYSTITIS (PBS/IC) Brian Birch, Tamsin Murray, Alan Cooper, and Bashir Lwaleed Brian BirchBrian Birch Southampton, United Kingdom More articles by this author , Tamsin MurrayTamsin Murray Southampton, United Kingdom More articles by this author , Alan CooperAlan Cooper Portsmouth, United Kingdom More articles by this author , and Bashir LwaleedBashir Lwaleed Southampton, United Kingdom More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.895AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Painful Bladder Syndrome/Interstitial cystitis (PBS/IC) is a debilitating condition of uncertain pathogenesis and a low treatment success. Honey has long been used for the treatment of wounds and has more recently been demonstrated to have beneficial effects on wound healing. The mechanisms include antibacterial properties, cytokine interaction and anti-oxidant effects. Mast cell activity appears to be an important mediator of inflammation in PBS/IC. It is postulated that honey has an effect on mast cell degranulation that would render it a potentially useful agent for intravesical use in this condition. METHODS The LAD2 mast cell line was used to study both spontaneous and provoked degranulation, measured as histamine release. The cells were pre-treated with a range of medicinal grade honeys and challenged with the mast cell activator Calcium ionophore A23187 and released histamine measured (by an ELISA assay). Control preparations included a clover nectar, a honey-like mixed sugar syrup and agents commonly used topically against PBS/IC (Uracyst™, Cystistat™, Gepan™, RIMSO-50™ and heparin). RESULTS Both spontaneous and A23187 induced histamine release were inhibited by honey in a dose dependent fashion from LAD2 cells. There were marked variations in this effect between honey types (between 60% and 100%). Syrup and clover nectar inhibited release by 40% and 36% respectively. Histamine release was not inhibited by Uracyst™, Cystistat™ or Gepan™ and only marginally by RIMSO-50™ and heparin; the maximum inhibition obtained by any drug at any dilution was 24%. CONCLUSIONS A constituent of most honeys inhibits spontaneous and stimulated mast-cell degranulation in a cell line model. This effect was not reproduced using other agents currently employed in clinical practice. Honey, therefore, has distinct potential as an intravesical agent both used alone and in combination. It warrants further investigation as a possible intravesical agent in the treatment of PBS/IC. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e381-e382 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Brian Birch Southampton, United Kingdom More articles by this author Tamsin Murray Southampton, United Kingdom More articles by this author Alan Cooper Portsmouth, United Kingdom More articles by this author Bashir Lwaleed Southampton, United Kingdom More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...