Abstract
To assess the clinically important mechanisms of drug resistance in breast cancer, we detennined the expression of the MRP and MDR1 genes in primary breast carcinoma specimens. Using RT-PCR, MRP RNA was detected in all specimens and MDR1 RNA in only 49 (51%) of the samples (n=97). Immunohistochemistry on frozen sections (n=30) was performed with monoclonal antibodies QCRL-1 and QCRL-3 (provided by Dr. S. Cole, Kingston) and C219, respectively. MRP staining was strong in 7 (23%) and weak in the remaining 23 (77%) specimens. Strong staining was more frequently observed in T3 &. T4 tumors than in T1 & T2 tumors, was present in the primary tumors of all 3 patients with distant metastases but was independent of histologic grade, estrogen receptor status and lymph node involvement. P-glycoprotein expression was seen in 15 (50%) specimens. Thus both the MRP and the MDR1 genes are frequently expressed in breast cancer and might be involved in the clinical drug resistance of this disease.
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