Abstract
In poorly controlled insulin-dependent diabetes, serum Sm-C concentrations are low despite high levels of growth hormone (GH), suggesting GH resistance. Whether this resistance might be due to receptor or post-receptor defects remains however unclear. Therefore, the number and affinity constant of liver bovine GH binding sites and the serum Sm-C concentrations, 24 h after injection of graded doses of GH, were determined in hypophysectomized rats injected with saline (controls) or with 40 mg of streptozotocin/kg BW (diabetics). After one week of diabetes there were no significant changes in the affinity constants (diabetics : 0.68 ± 0.04 × 109 M−1 vs controls : 0.92 ± 0.07 × 109M−1 ; mean ± SE; n=8; P < 0.1) or the numbers of GH binding sites (diabetics : 1.10 ± 0.12 pmol/mg DNA vs controls : 1.26 ± 0.15 pmol/mg DNA; P < 0.5). Despite the absence of alterations in GH binding, the maximal serum Sm-C response was severely blunted in the diabetics (2.02 ± 0.32 U/ml) when compared to controls (17.41 ± 2.69 U/ml; P < 0.01). The sensitivity (ie ED50) however was the same for control and diabetics rats.
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