Abstract
The aim of this study was to count the total sum of cellulolitic bacteria colony in aceh cattle rumen. The sample used was aceh cattle rumen liquid which taken from salughtered house in Banda Aceh. Aceh cattle rumen liquid were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Petri dish was entered to candle jar to be incubated in an incubator with temperature 37oC about 96 hours. Colony of cellulolitic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of colony), counting total of bacteria which was grown, and Gram staining. The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil. The average number bacteria of total bacteria in the cattle rumen liquid was 4,81 x 105 cfu/ml
Highlights
Colony of cellulolitic bacteria which were grown were observed by using colony bacteria criteria, counting total of bacteria which was grown, and Gram staining
The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil
Journal of Ichthyology and AquaticBiology. 9:161-176. Singh, S., V.S. Moholkar. and A. Goyal. 2013. Isolation, identification and characterization of a cellulolytic bacillus amyloliquefaciens strain SS35 102. J. Kaunia. 10 (2): 92-102. Ulrich, A, G. Klimke and S. Wirth. 2008. Diversity and activity of cellulosedecomposing bacteria, isolated from a sandy and a loamy soil after long-term manure application. Microb Ecol 55: 512–522. Press, Malang
Summary
Koloni yang tumbuh pada masing-masing media BHM-CMC agar dari pengenceran 10-2 sampai pengenceran 10-5 akan dihitung seluruhnya. Setelah diperoleh jumlah koloni dari setiap pengenceran, selanjutnya dihitung total bakteri selulolitik dengan cara mengalikan jumlah koloni dengan satu per faktor pengenceran yang dipakai. Jumlah koloni yang digunakan untuk menghitung total bakteri selulolitik adalah koloni yang berjumlah antara. Satuan yang digunakan untuk penghitungan jumlah bakteri adalah cfu/ml. Jumlah total bakteri per ml dapat dihitung dengan menggunakan rumus sebagai berikut : Jumlah total bakteri = Jumlah koloni ×
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