Abstract
The first report of silencing in the vegetative phase of growth in fungi was made in Neurospora crassa. A loss of hygromycin resistance was observed as a result of transformation with a plasmid carrying the bacterial hygromycin phosphotransferase ( hph ) gene, fused to the promoter of the trpC gene of Aspergillus nidulans. Growth of Neurospora crassa in the presence of the cytosine analog 5-azacytidine that prevents cytosine methylation, resulted in the reactivation of the hph gene, suggesting that DNA methylation is involved in gene silencing. When the silencing of both transforming DNA and homologous endogenous sequences was observed in Neurospora crassa —it was termed “quelling.” Evidence that quelling is a general mechanism, not limited to specific genes in Neurospora, was provided by the fact that the qa-2 gene encoding the quinic acid dehydrogenase used as a genetic marker for transformation in experiments was also silenced. As well as being dominant, it was also demonstrated that quelling in Neurospora is a posttranscriptional mechanism.
Published Version
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