Abstract
Kankakee mallow is an endangered herbaceous perennial that is indigenous to Kankakee County, Illinois. Stock plants were from seeds pretreated in 82°C water prior to greenhouse germination and growth. Nodal explants were disinfested and placed in vitro onto agar-solidified MS medium containing 0, 0.1, 1.0, 5.0, or 10.0 μM BA and 1.0 μM IBA. Axillary shoots grew and elongated best when the medium contained no cytokinin. BA tended to result in a rosette pattern of leaves. Within hours of placing the original explants in vitro and shortly after subsequent transfers were made (even when there was no cutting) a bright pink exudate appeared in the medium. The most vigorous cultures tended to form the most exudate. Microshoots were placed in a high humidity container in vermiculite wetted with water. Rooting was 50% without auxin. Plants were transplanted into pots containing peat-lite medium and successfully acclimatized to the greenhouse.
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