Abstract
Micropropagation has been used to rescue Leontochir ovallei, an endangered Chilean species in the Alstroemericeae. Cultures were initiated by aseptically germinating seeds of Leontochir on a medium containing 1/10 MS salts and vitamins and 0.3% sucrose. Three types of cytokinins (BAP, 2-iP and kinetin) at four concentrations (0, 2, 4, and 8 uM) were studied for shoot proliferation. In the 4 uM BAP treatment, new shoots were produced at an average of six per culture after four weeks of culture. Overall, there was an average of four shoots/culture/4 weeks for all BAP treatments. This was significantly higher than the 2-iP and kinetin treatments. Moreover, the increase of culture fresh weight over time was significantly greater in BAP treatments than those in other treatments. A rooting study compared the effect of NAA and IBA on root initiation. Over 85% of the cultures in 10 and 20 uM NAA treatments produced healthy and large roots. This was significantly higher than the 10 and 20 uM IBA treatments. In summary, a concentration of 4 uM BAP combined with 1 uM IBA in MS salts and vitamins supplemented with 146 mg glutamine/l is the best for shoot proliferation of leontochir; an MS basal medium containing 10 uM NAA is the best for root initiation. Micropropagated plantlets have been successfully transplanted into the greenhouse for further genetic and breeding studies.
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