875 PRIMARY CULTURE OF HUMAN BLADDER TUMOR BY CANCER TISSUE-ORIGINATED SPHEROID

Abstract

You have accessJournal of UrologyBladder Cancer: Basic Research1 Apr 2011875 PRIMARY CULTURE OF HUMAN BLADDER TUMOR BY CANCER TISSUE-ORIGINATED SPHEROID Kazuo Nishimura, Hiroaki Okuyama, Ken Takeda, Takahiro Yoshida, Yasuyuki Arai, Masashi Nakayama, Kenichi Kakimoto, and Masahiro Inoue Kazuo NishimuraKazuo Nishimura Osaka, Japan More articles by this author , Hiroaki OkuyamaHiroaki Okuyama Osaka, Japan More articles by this author , Ken TakedaKen Takeda Osaka, Japan More articles by this author , Takahiro YoshidaTakahiro Yoshida Osaka, Japan More articles by this author , Yasuyuki AraiYasuyuki Arai Osaka, Japan More articles by this author , Masashi NakayamaMasashi Nakayama Osaka, Japan More articles by this author , Kenichi KakimotoKenichi Kakimoto Osaka, Japan More articles by this author , and Masahiro InoueMasahiro Inoue Osaka, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.699AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Development of three-dimensional (3D) culture models that mimic original tumor biology has a crucial role in evaluation of drug efficacy in vitro and subsequent clinical trials. Here we report a novel 3D culture system named caner tissue-originated spheroid (CTOS). The CTOSs derived from human bladder tumors were characterized and assayed for drug sensitivity. METHODS Bladder tumor tissue samples were collected by transurethral resection of bladder tumor (TUR-Bt) or biopsy, followed by mechanical and enzymatic digestion. Organoid fraction, which was retained in the filter, was collected and cultured with stem cell medium for a couple of days to observe formation of CTOS. Morphology, cell characters and growth activity of CTOS were compared with primary tumors by HE staining, immunohistochemical studies with E-cadherin, α-smooth muscle actin (SMA), p53 and Ki67. Growth assay of CTOS was applied for assessing CDDP chemosensitivity. RESULTS CTOS was able to be isolated from 69 cases (68%) out of 101 cases. HE staining showed that the morphology of CTOS was similar with that of primary tumors. CTOS was composed of E-cadherin positive epithelial cells excluding α-SMA positive myofiblastic stromal cells. Staining pattern of p53 was coincident between CTOS and primary tumors. Ki67 expression in primary tumors was correlated with growth activity of CTOS. Growth inhibition of individual CTOS after exposure to CDDP was diverse. The growth inhibitory effects were dose-dependent of CDDP in CTOS from urothelial carcinoma (UC) cases, while CTOS from a squamous cell carcinoma (SCC) case was resistant to CDDP. CONCLUSIONS CTOS is a highly viable spheroid derived from highly purified human cancer cells. CTOS can be isolated from a majority of bladder tumor cases. The growth activity of CTOS reflects that of primary tumors. CTOS is applicable to chemosensitivity assay. Further investigation would clarify the usefulness of CTOS for clinical application of bladder tumor. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e350 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kazuo Nishimura Osaka, Japan More articles by this author Hiroaki Okuyama Osaka, Japan More articles by this author Ken Takeda Osaka, Japan More articles by this author Takahiro Yoshida Osaka, Japan More articles by this author Yasuyuki Arai Osaka, Japan More articles by this author Masashi Nakayama Osaka, Japan More articles by this author Kenichi Kakimoto Osaka, Japan More articles by this author Masahiro Inoue Osaka, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...