87-P

Abstract

Aim Characterization and identification of new HLA alleles have been important contributions to bone marrow and hematopoietic stem cell transplantation (BM/HSCT) for optimal donor selection which in turn will improve graft outcome. They are also invaluable in solid organ transplantation for selecting compatible donors according to organ transplant recipients’ antibody profiles. Methods Routine HLA typing by sequence specific oligonucleotide (SSO) probe based techniques and unusual or potential new variant of HLA alleles were typed by sequence based method in which alleles at a locus were separated by sequence specific primers and then sequenced. Results Four new HLA alleles have been identified and characterized in a donor and three BM/HSC transplant recipients. The profiles of these new alleles are shown in Table 1. Conclusions The B ∗ 40:206 and C ∗ 02:58 was identified in Hispanic male patients; DQB1 ∗ 03:40 in a Caucasian male donor; and the DQB1 ∗ 06:50 in a Black patient. Finding a matched donor for patients bearing these alleles would be difficult and challenging in BM/HSCT. Patient may make antibody to these alleles which in turn would impact graft outcome in solid organ transplantation. Therefore, the identification of these new alleles is important addition to the HLA allele list. Further investigation of the transplant outcome of these patients bearing these new alleles would be warranted [Fig. 1]. Download full-size image