8636 Metabolic Profiling of the Endometrium in Patients with Recurrent Uterine Myoma.

Abstract

<h3>Study Objective</h3> Identification of biomarkers for recurrent uterine fibroids based on metabolomic analysis of the endometrium. <h3>Design</h3> Level II Canadian Task Force. <h3>Setting</h3> Operative Gynecology Department, National Medical Institute of Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Moscow, Russia. <h3>Patients or Participants</h3> 35 patients with newly diagnosed uterine fibroids (UF) and 31 patients diagnosed with recurrent uterine fibroids (RUF) underwent laparoscopic myomectomy. 15 patients in the comparison group (CG)underwent intrauterine septum resection. <h3>Interventions</h3> Endometrial biopsy was performed in the 1st phase of the menstrual cycle during the surgery. Histopathological examination and lipidomic analyses of the specimens took place. Qualitative and quantitative assessment of lipidoma was performed using electrospray mass spectrometry. <h3>Measurements and Main Results</h3> Statistically significant differences in lipidomic status of the compared clinical groups were revealed. The lowest level of lipids of the following classes was noted in patients with RUF: ceramides, sphingomyelins, phosphatidylserines (exceptions - PS 34:0, PS 36:0, PS 42:5, PS 46:1), significant phosphatidylethanolamines (exceptions - PE 44:2, PE 38:3, PE 42:6, PE 44:6, PE O-38:3, PE O-38:4), phosphatidylcholines (exceptions - PC 38:5, PC 36:4, PC O-38:1, PC O-38:0, PC O-38:2, PC 38:2, PC 40:0), phosphatidylglycerols with a total chain length of less than 40 carbon atoms. The highest level of lipids of the following classes was noted in RUF when comparing with lipidomic data of patients with UF and CG: lysophosphatidylcholines, phosphatidylglycerols with a total chain length of more than 40 carbon atoms. <h3>Conclusion</h3> Analysis of the lipid composition of the endometrium revealed ceramides, sphingomyelins, phosphatidylserines, phosphatidylethanolamines, phosphatidylcholines, the levels of which differ significantly in endometrial tissues of patients with RUF as compared with patients in uterine fibroids and comparison groups. This data opens new possibilities for lipidomic-based analysis of the endometrium for prediction of recurrence of uterine fibroids.