862 RashX: Immune single-cell transcriptional classification of human chronic inflammatory skin disease

Abstract

We have developed one of the largest single-cell transcriptomic datasets of CD45+ immune cells in human inflammatory skin disease, including 13 psoriasis and 11 atopic dermatitis samples. Our analyses identify skin-resident memory T cells as containing the primary molecular signature differentiating psoriasis and atopic dermatitis, composed of 78 transcripts with disease specificity at a p value < 0.001, only 13 of which have been previously reported. Recomposition into pseudo-bulk transcriptomes diminishes discriminative significance of all but 2 of these genes, demonstrating the critical role of single-cell approaches in discriminating disease states.