845 CEACAM1 DISTRIBUTION AND ITS ROLE IN LYMPH NODE METASTASIS IN PENILE CANCER

Abstract

You have accessJournal of UrologyPenis/Testis/Urethra: Benign & Malignant Disease III1 Apr 2010845 CEACAM1 DISTRIBUTION AND ITS ROLE IN LYMPH NODE METASTASIS IN PENILE CANCER Derya Tilki, Boris Schlenker, Oliver Reich, Michael Seitz, Christian Stief, and Süleyman Ergün Derya TilkiDerya Tilki Munich, Germany More articles by this author , Boris SchlenkerBoris Schlenker Munich, Germany More articles by this author , Oliver ReichOliver Reich Munich, Germany More articles by this author , Michael SeitzMichael Seitz Munich, Germany More articles by this author , Christian StiefChristian Stief Munich, Germany More articles by this author , and Süleyman ErgünSüleyman Ergün Essen, Germany More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.2347AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Squamous cell cancer of the penis is a rare malignancy which has a high preference to metastasize to regional inguinal lymph nodes. Early detection of lymph node disease is critical for clinical outcome. Carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) has been shown to be involved in tumor angiogenesis and lymphangiogenesis. To evaluate the role of CEACAM1 in squamous cell penile cancer we studied the expression of CEACAM1 in primary tumor and lymph node tissues. METHODS Immunohistochemistry for CEACAM1 and CD34 has been performed on penile cancer tissue (n=21) and lymph node tissue with and without histologically proven penile cancer metastasis (n=20). RESULTS Based on the vascularization pattern of tumor tissue visualized by immunostaining for CD34 on penile cancer tissue, we distinguished two tumor types. In a part of the penile cancer cases, blood vessels were almost only in connective tissue septa surrounding tumor cell clusters. Here, blood vessels were CEACAM1 negative, but a subset of tumor cells, which was organized around keratinized penile cancer areas, showed positive CEACAM1 staining. In contrast, the second tumor type was highly vascularized and showed solidly organized tumor tissue without strong connective tissue septa. Interestingly, the great majority of tumor tissue in these tumors was negative for CEACAM1, only few small tumor cell clusters organized exhibited CEACAM1 staining. In contrast, no CEACAM1 staining was found in the normal penile epidermis. In positive lymph nodes strong staining for CEACAM1 was found in cells lining the sinusoidal spaces of the lymph node which were partially enlarged apparently prior to the arrival of tumor cells. Remarkably, in lymph nodes with tumor cell clusters mainly tumor cells invading the lymphatic tissue and/or the outer row of tumor cells exhibited CEACAM1 staining. Tumor cell clusters within the lymphatic tissue were found not to be vascularized. CONCLUSIONS Our data show that only a subset of sqamous cell penile cancer cells exhibit CEACAM1 while no CEACAM1 staining was found in penile epidermis and blood vessels of penile cancer. Since tumor cells metastasized to the lymph nodes and endothelial cells lining the sinusoids of these lymph nodes were positive for CEACAM1, we hypothesize that homophilic interaction between CEACAM1 might be involved in the lymph node metastasis of penile cancer. Further studies are needed in order to evaluate the clinical relevance of CEACAM1 in this cancer entity. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e330 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Derya Tilki Munich, Germany More articles by this author Boris Schlenker Munich, Germany More articles by this author Oliver Reich Munich, Germany More articles by this author Michael Seitz Munich, Germany More articles by this author Christian Stief Munich, Germany More articles by this author Süleyman Ergün Essen, Germany More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...