Abstract
The objective was to study whether the high erythropoietic stimulatory activity in plasma from neonatal mice is erythropoietin (Ep) alone or Ep in combination with other factors. Plasma from hypertransfused (hy.tr.) neonatal (20d) and adult (13-20w) WLO-mice were compared by a RIA and a cell culture assay for Ep. The bioassay reflects erythropoiesis stimulating factor(s) (ESF), defined as the net activity of Ep and other stimulatory and possible inhibitory factors. The RIA determines immunoreactive Ep (iEp). There was no difference between the mean iEp levels of hy. tr. neonatal and adult animals (P>0.3). ESF was not detectable in hy.tr. adult mice, while significant levels were found in neonatal animals. Thus, the mean ESF level of hy.tr. neonatal mice was significantly above that of adult animals (P<0.001). The data show that plasma from hy.tr. neonatal mice contain one or more erythropoietic stimulatory factors not detected by the RIA for Ep. It is concluded that part of the high erythropoietic stimulatory activity in plasma from neonatal mice is due to non-Ep factors.
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