Abstract
Affinity chromatography of lysozyme has been carried out using a variety of adsorbents: dispersed chitin, carboxymethylated chitin, deaminated chitin, and chitin-coated cellulose. This chapter describes the preparation and use of an affinity adsorbent based on a low molecular weight substrate analog of lysozyme. The procedure for preparation of the adsorbent is general and could be used for coupling other saccharides to agarose. The experiments described in this chapter were carried out in order to determine optimum elution conditions for handling small amounts of lysozyme such as are manipulated during purification of synthetic protein.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
