825 Aquaphilus dolomiae S0, a thermal spring water active compound, showing broad repairing properties of on in vitro models of injured skin

Abstract

The clinical efficacy of Avène thermal spring water in atopic dermatitis, psoriasis or in reducing erythema post laser resurfacing have been shown by several studies. In addition to these soothing and immunomodulatory properties, in-vitro experiments have also demonstrated effects of Avène thermal spring water on stimulation of keratinocyte differentiation and improvement of membrane fluidity, suggesting a potential effect on skin barrier and repair. An investigation of the deep aquifer of the Avène thermal spring water pointed out a new microorganism as a potential source of these unique properties. Based on its distinctive phenotypic and genotypic characteristics, this newly identified strain was assigned to a new genus, as a representative of a novel species called Aquaphilus dolomiae. It is a chemoorganotrophic non-spore-forming bacterium of the b-Proteobacteria class. In the present study, the activity of S0, an original biological extract of A. dolomiae, was evaluated on in-vitro models of injured skin. The compound showed positive properties on primary fibroblast proliferation and keratinocyte migration. When formulated, it favored skin re-epithelialization on a 3D model of wounded skin explants. Moreover, we showed that S0 could prevent wound infection by up-regulating numerous antimicrobial peptide genes and inducing hBD2 peptide release. All together, these results show broad repairing properties of the A. dolomiae extract S0, helping skin repair and preventing complicated wounds.