82 CRYOABLATION INDUCED ALTERATIONS OF PORCINE RENAL ARTERIAL ANATOMY AND BLOOD FLOW

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research I1 Apr 201082 CRYOABLATION INDUCED ALTERATIONS OF PORCINE RENAL ARTERIAL ANATOMY AND BLOOD FLOW Brunolf Lagerveld, Pepijn van Horssen, Pilar Laguna Pes, Jeroen van den Wijgaard, Hessel Wijkstra, Jos Spaan, and Jean de la Rosette Brunolf LagerveldBrunolf Lagerveld More articles by this author , Pepijn van HorssenPepijn van Horssen More articles by this author , Pilar Laguna PesPilar Laguna Pes More articles by this author , Jeroen van den WijgaardJeroen van den Wijgaard More articles by this author , Hessel WijkstraHessel Wijkstra More articles by this author , Jos SpaanJos Spaan More articles by this author , and Jean de la RosetteJean de la Rosette More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.130AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The time frame in which the vascular response of cryoablated tissue is completed remains unclear. The aim of this study is to investigate in vivo the anatomical changes of arterial structures and their blood flow of porcine normal renal parenchyma after cryosurgery. METHODS Ten anesthetized pigs underwent 20 minutes of focal cryoablation of both kidneys. All kidneys were divided into 5 groups with survival time of 20 min, 4 hrs, 2, 7 and 14 days. Before kidney removal, microspheres were intra-arterially administrated. After harvesting, the main renal artery was flushed with saline and filled with fluorescence dyed replica material. Each kidney was frozen and processed in an imaging cryomicrotome resulting in 2 stacks of images of 2000x2000 pixels. The 1st stack resulted in a 3D image of the arterial structure, the 2nd stack in a 3D microsphere distribution matching the arterial anatomy. The arterial vasculature was segmented and vessel diameters were estimated using a combination of filtering and a peeling algorithm. Thereafter, all vessel segments could be classified according to their diameters that were divided over bins of 4 microns wide starting from 40 μm to 500 μm. The length of all segments of a specific bin diameter was added and histograms of total summed vessel length as function of diameter were plotted. The histograms for the ablated area were compared to the histograms from a control area in a different pole. RESULTS In the 20 minutes group, vascular structures with diameter > 200 μm remained anatomically intact in the ablated area but microspheres passing through are only found in surrounding tissue and are not distributed in the ablated area. Remaining vascular structures disappeared over time in the ablated zone. After 2 weeks this process was completed as shown in the histograms per group (figure 1). CONCLUSIONS The acute injury induced by cryoablation starts with the destruction of arteries smaller than 200 μm leaving the structures of larger vessels intact and allowing blood flow to the surrounding tissue. After 2 weeks the vessel destruction in the ablated zone is complete and blood flow has fully ceased. Amsterdam, Netherlands© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e34 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Brunolf Lagerveld More articles by this author Pepijn van Horssen More articles by this author Pilar Laguna Pes More articles by this author Jeroen van den Wijgaard More articles by this author Hessel Wijkstra More articles by this author Jos Spaan More articles by this author Jean de la Rosette More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...