815 MOLECULAR STUDY OF PTEN AND SMAD4 GENES ON UROGENITAL SYSTEM OF THE RATS IN DIFFERENT AGES

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research (I)1 Apr 2013815 MOLECULAR STUDY OF PTEN AND SMAD4 GENES ON UROGENITAL SYSTEM OF THE RATS IN DIFFERENT AGES Beatriz Rocha, Sicília Colli, Leilane Nepomuceno, Diogo Souza, Bianca Gregório, Carla Gallo, and Francisco Sampaio Beatriz RochaBeatriz Rocha Rio de Janeiro, Brazil More articles by this author , Sicília ColliSicília Colli Rio de Janeiro, Brazil More articles by this author , Leilane NepomucenoLeilane Nepomuceno Rio de Janeiro, Brazil More articles by this author , Diogo SouzaDiogo Souza Rio de Janeiro, Brazil More articles by this author , Bianca GregórioBianca Gregório Rio de Janeiro, Brazil More articles by this author , Carla GalloCarla Gallo Rio de Janeiro, Brazil More articles by this author , and Francisco SampaioFrancisco Sampaio Rio de Janeiro, Brazil More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.381AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Cancer is increasing in low- and middle-income countries and it is a major public health problem in many parts of the world. Genes PTEN and Smad4 are involved in many cellular processes such as cell proliferation, migration, cell survival and apoptosis. Levels of Smad4 are increased in Gleason score 3-4 and are reduced in proportion to the increase of Gleason score. Recent studies showed that the combination of PTEN and Smad4 genes resulted in a high efficiency as a predictive tool of patient survival. We aimed to identify the expression of PTEN and Smad4 upon urogenital system organs of Wistar rats at different ages. METHODS The study was approved by the animal care and use committee of the Local Biology Institute (021/2012). Males and females wistar rats were separated into 3 groups, according to the age group corresponding: 21days (males, n=10 and females, n=5), 50 days (males, n= 5 and females, n=5) and 90 days (males, n=10 and females, n=5). All animals were killed with a lethal dose of pentobarbital. Bladder (males and females), ventral prostate, testis, ovary and uterus were removed and stored at −80°C for RNA extraction. Total RNA from tissue was extracted using trizol reagent, then 1 ug RNA was reverse transcribed by Superscript III to obtain cDNA. cDNA was used to evaluate the gene expression of PTEN and Smad4 by real time PCR using SYBR GreenER. The reactions were normalized by β-actin gene expression. Data were analyzed by one-way ANOVA and Bonferroni post-test, considering p<0.05. RESULTS Concerning PTEN gene expression in different ages no difference was found on bladder and prostate in male rats. On the other hand, the group of 90 days showed an increase of 80% in its expression on testis when compared to the 21 days group (p = 0.02). In different ages of females, PTEN gene expression did not vary on bladder and ovaries. As for gene expression of Smad4 in males, there were no differences in prostate and bladder among the groups. However, its expression was reduced on testis at 50 and 90 days (93% and 83%, respectively) when compared to the 21 days (p = 0.0005). As for females, the gene expression of Smad4 in bladder did not differ among different ages. Concerning ovary, the authors observed a reduction of 73% and 59% in its expression at 50 and 90 days, respectively, compared to the 21 days (p = 0.0030). CONCLUSIONS Thus, the increase in the expression of PTEN to 90 days can indicate a connection between the gender and its entry into puberty. Similarly, a reduction in the expression of Smad4 to 50 and 90 days seems to show an important role in the early development of the folicular and testicular development. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e335 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Beatriz Rocha Rio de Janeiro, Brazil More articles by this author Sicília Colli Rio de Janeiro, Brazil More articles by this author Leilane Nepomuceno Rio de Janeiro, Brazil More articles by this author Diogo Souza Rio de Janeiro, Brazil More articles by this author Bianca Gregório Rio de Janeiro, Brazil More articles by this author Carla Gallo Rio de Janeiro, Brazil More articles by this author Francisco Sampaio Rio de Janeiro, Brazil More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...