814 Polymorphism of Surfactan Protein B Genes and the Risk of Respiratory Distress Sendrome and Chronic Lung Disease in Preterm Neonates

Abstract

Objectives: To determine whether SP-B iΔ4 gene polymorphism is associated with respiratory distress syndrome (RDS) and chronic lung disease (CLD) etiology. Method: This prospective and retrospective study was performed in the neonatal intensive care unit in Turkey. In order to identify SP-B iΔ4 gene polymorphism, we analysed genomic DNA by polymerase chain reaction. Preterm neonates with a gestational age below 34 completed weeks, who were treated in our unit were included the study. The neonates with congenital anomalies and the neonates who died before the postnatal 28th day were excluded. The study was approved by the Local Committee on Investigations in Human Subjects. Total of 131 preterms and 50 healthy term infants were investigated. Premature babies were divided into 4 with respect to the development of RDS and CLD. Results: The frequency of SP-B iΔ4 gene variations did not differ between preterms (10.7%) and terms (10%). A total of 117 preterms with SP-B iΔ4 gene wild type (group A) and 14 preterms carrying the genetic variations (group B) did not differ in gestational age, gender distributionn and birth weight, RDS and CLD. In premature study supgroups, SP-B iΔ4 gene variations was 10.7% in control group (n:80), 15.8% in RDS group (n:19), 10% in RDS and CLD group (n:20) and 0% in CLD group (n:8). There wasn't any statistically significant difference between all supgroups. Conclusions: In our premature babies, we coulnd't show any association between SP-B iΔ4 gene polymorphism and RDS / CLD.