Abstract
Background: Fluorescence Imitating Brightfield Imaging (FIBI) is a novel alternative microscopy method that can image freshly excised, non-sectioned tissue.We examine its potential utility in dermatopathology. Methods: Five skin samples embedded in paraffin blocks were superficially deparaffinized using xylene and ethanol and then briefly stained with hematoxylin and eosin (H&E). FIBI captured tissue surface histology images using simple microscope optics and a color camera. Subsequently, we applied deep learning-based models to improve resemblance to standard H&E coloration and contrast.
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