810: Aberrant O-linked glycosylation in preeclampsia

Abstract

urine specimens from normal pregnant women and from chronic hypertension. In PE, urinary nephrin and podocalyxin concentrations were significantly higher than those in normal pregnancy and chronic hypertension (p 0.01), and their levels were highly correlated to each other, y 4.611x 327,491, r2 0.595. Nephrin and podocalyxin with urine protein concentrations were also highly correlated, y 257.354x 297.898, r2 0.746 for nephrin and y 31.115x 10.640, r2 0.390 for podocalyxin, respectively. ig-h3 was detected in the urine specimens from PE but undetectable in normal pregnancy and pregnancy complicated with chronic hypertension. In PE, urinary ig-h3 levels were also highly correlated to nephrin and podocalyxin concentrations, y 117.069x 260.337, r2 0.515 for nephrin and y 18.571x 41.694, r2 0.463 for podocalyxin. CONCLUSION: These results provide strong evidence that specific podocyte protein shedding is associated with glomerular barrier dysfunction in PE. Urinary ig-h3 excretion indicates that increased TGFactivity might be involved in the pathology of renal dysfunction in PE. Nephrin, podocalyxin, and ig-h3 could serve as surrogate biomarkers of glomerular barrier dysfunction in PE.