81] Thin-layer chromatography and thin-layer electrophoresis of vitamin B6

Abstract

Publisher Summary The advantages of thin-layer chromatography and thin-layer electrophoresis are well documented. The thin-layer chromatography of vitamin B6 has been the subject of several studies. Thin-layer electrophoresis has been applied to the separation of compounds in the vitamin B6 group. Separation of the nonphosphorylated forms of vitamin B6 (pyridoxol, pyridoxal, pyridoxamine, and the most important metabolites, 4-pyridoxic acid and its lactone) involves problems different from those created by separation of the phosphorylated forms, and hence the two classes of compounds are discussed individually. When absorbents containing fluorescence indicators are used, all forms and derivatives of vitamin B6 can be detected through the quenching of fluorescence in ultraviolet light. Thin-layer electrophoresis adds another important possibility to separation techniques and can be utilized either in conjunction with thin-layer chromatography as a second dimension or independently of it in cases where thin-layer chromatography does not offer sufficient separation.