Abstract
BackgroundGastric cancer is one of the most common fatal disease worldwide, but its mechanism and therapeutic targets are still unclear. In this study, we have analyzed the differences in gene modules and key pathways in gastric cancer patients, and elaborated the mechanism and effective treatment of gastric cancer with microarray data from the gene expression omnibus (GEO) database. MethodsGEO2R tools was used to identify differential expression genes (DEGs) and String database was employed to construct protein-protein interaction (PPI) network. We firstly imported the PPI network into the Cytoscape software to find key nodes, and then employed the DAVID database to enrich and annotate the pathways and key modules. Results63 characteristic genes of gastric cancer are involved in regulation of ECM-receptor interaction, focal adhesion and Protein digestion and absorption. SPARC, FN1, BGN and COL1A2 are four key nodes relating to tumour proliferation and metastasis, and their expression were strongly associated with poor survival (p<0.05). 13 transcription factors including PRRX1 have remarkable changes in gastric cancer. ConclusionsThe crucial genes and pathways of gastric cancer were explored through different perspectives via multiple bioinformatics methods. Our study will not only contribute to elucidating the pathogenesis of gastric cancer, but also provide prognostic markers and potential therapeutic targets for gastric cancer. Legal entity responsible for the studyThe authors. FundingNational Natural Science Foundation of China (No. 81503165), Natural Science Foundation of Zhejiang Province (No. Q17H310010). DisclosureAll authors have declared no conflicts of interest.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
