804 IL-17 EXPRESSED LEUKOCYTES ARE CHARACTERISTIC OF PROSTATE INFLAMMATORY ATROPHY LESIONS IN PATIENTS WITH PROSTATE CANCER

Abstract

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Prostate & Genitalia1 Apr 2010804 IL-17 EXPRESSED LEUKOCYTES ARE CHARACTERISTIC OF PROSTATE INFLAMMATORY ATROPHY LESIONS IN PATIENTS WITH PROSTATE CANCER Eugene Vykhovanets, Gregory MacLennan, Olena Vykhovanets, and Sanjay Gupta Eugene VykhovanetsEugene Vykhovanets More articles by this author , Gregory MacLennanGregory MacLennan More articles by this author , Olena VykhovanetsOlena Vykhovanets More articles by this author , and Sanjay GuptaSanjay Gupta More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1480AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The phenotype of intraprostatic mononuclear cells (MNC) is still illusive, despite their role for chronic inflammation and prostate cancer (PCa). Expression of interleukin (IL)-17 is a characteristic of a CD4 T cell subset called T helper 17 (TH17) cells. Their role in host defense and in autoimmunity was recently established. Little is known about other IL-17 expressing cells at sites of tissue inflammation. Based on our previous findings we hypothesized that the IL-17 producing cells may be targets of specific inflammatory signals that initiate inflammation at sites of proliferative inflammatory atrophy (PIA) of the prostate. METHODS To immunophenotype MNC infiltrates we evaluated 30 formalin-fixed paraffin-embedded whole mount radical prostatectomy specimens. Two-color immuno-histochemestry was employed to distinguish macrophages (Mφ), B, T, and CD4 T helper cells. Quantitation of IL-17 cells was performed in all sites of MNC accumulation in the entire prostate, noting their relationships to PCa, PIA, or benign tissue. RESULTS T and B cells were commonly observed at the site of MNC accumulation (“hot spots”). The number of hot spots varied significantly from case to case (mean = 24±12 [SD] per prostate). MNC aggregates were found in and around areas of PCa, and in areas of PIA. The numbers of B and T cells were almost equal within the MNC hot spots. CD4 helper cells were the predominant T cells in the MNC hot spots. Levels of IL-17 cells were similar in zones of benign prostate tissue and areas of PCa. Pronounced intraluminal and peri-glandular IL-17 cell accumulation characterized the MNC infiltrates associated with PIA lesions. The number of IL-17 cells in the intraluminal and peri-glandular areas of PIA lesions was 5 fold higher than in the MNC aggregates within areas of PCa and 5 fold higher than in benign tissue populated by only scattered MNC. The number of IL-17 cells in the intraluminal and peri-glandular areas of PIA lesions was 3 fold higher than in MNC aggregates in benign tissue adjacent to PCa, and 3 fold higher than in randomly situated MNC aggregates in benign prostate tissue. Consecutive slide analysis indicated that glandular and peri-glandular Mφ and neutrophils, rather than TH17 cells, were the predominant IL-17 producing cells in PIA lesions. CONCLUSIONS These data demonstrate that release of inflammatory IL-17 by MNC may be the initiating factor in prostatic PIA. The accumulation of IL-17 producing cells in PIA lesions presents direct evidence of an inflammatory microenvironment that may support the development and progression of PCa. Cleveland, OH© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e314 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Eugene Vykhovanets More articles by this author Gregory MacLennan More articles by this author Olena Vykhovanets More articles by this author Sanjay Gupta More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...