802 NAPROXEN AND MORPHINE REVERSES BLADDER OVERACTIVITY INDUCED BY BLADDER ACETIC ACID INFUSION IN ANESTHETIZED FEMALE RATS

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research1 Apr 2011802 NAPROXEN AND MORPHINE REVERSES BLADDER OVERACTIVITY INDUCED BY BLADDER ACETIC ACID INFUSION IN ANESTHETIZED FEMALE RATS Veronique Guilloteau, Karine Rollet, Marc Guerard, Timothy Westfall, Stefano Palea, and Philippe Lluel Veronique GuilloteauVeronique Guilloteau Toulouse, France More articles by this author , Karine RolletKarine Rollet Toulouse, France More articles by this author , Marc GuerardMarc Guerard Toulouse, France More articles by this author , Timothy WestfallTimothy Westfall Toulouse, France More articles by this author , Stefano PaleaStefano Palea Toulouse, France More articles by this author , and Philippe LluelPhilippe Lluel Toulouse, France More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.620AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Bladder overactivity (OAB) is characterized by an increase in micturition frequency (MF). Bladder acetic acid (AA) infusion is a common animal model used to produce OAB. This study evaluated the effects of anti inflammatory and analgesic agents in OAB model in anesthetized rats. METHODS Rats were anesthetized with urethane. Catheters were implanted into the bladder and jugular vein for intravesical pressure recording and drug administration, respectively. Saline was infused into the bladder for 45 min followed by AA for 30 min. The effects of naproxen, 3 mg/kg, intravenously i.v. (n=8), morphine, 3 mg/kg, subcutaneously s.c. (n=8) or vehicle (i.v. n=5 and s.c. n=5) were followed during 60 min post-administration. Intercontraction interval (ICI) and threshold pressure (ThP) were calculated for two 30 min periods. MF was calculated by 15 min periods. For each parameter, comparison between control value (bladder saline infusion) and basal values (bladder AA infusion) was performed using paired Student t-test and the effects of compounds were compared to basal values (bladder AA infusion) using a one way Anova with repeated measures followed by Newman-Keul's test. RESULTS Intravesical AA induced a significant decrease in ICI (212±26 sec) compared to saline (403±38 sec) (pooled data). Moreover AA bladder infusion significantly increased MF frequency in each group. All other cystometric parameters were not modified by AA 0.2% bladder infusion. Vehicle did not modify cystometric parameters whereas naproxen and morphine significantly reversed the effect of AA on ICI 0-60 min post-administration (figure 1). MF was significantly decreased after naproxen (1.6 ± 0.4 versus 4.7 ± 0.6 peaks/15 min) and morphine (1.3 ± 0.4 versus 6.4 ± 1.0 peaks/15 min) administrations compared to basal AA values. Morphine (3 mg/kg, s.c.) significantly increased ThP compared to basal values with a maximum effect 30–60 min post-administration (11 ±1 versus 8 ± 1 mmHg). CONCLUSIONS Intravesical AA in female rats elicited OAB characterized by an increase in MF. Naproxen, a non steroid anti inflammatory drug and morphine, an analgesic agent, are able to reverse the OAB induced by bladder AA infusion. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e323 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Veronique Guilloteau Toulouse, France More articles by this author Karine Rollet Toulouse, France More articles by this author Marc Guerard Toulouse, France More articles by this author Timothy Westfall Toulouse, France More articles by this author Stefano Palea Toulouse, France More articles by this author Philippe Lluel Toulouse, France More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...