800 DEGARELIX REDUCES URODYNAMIC CHANGES IN A RAT MODEL FOR EXPERIMENTAL DETRUSOR OVERACTIVITY AND PRODUCES MORE EFFICIENT VOIDING

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research1 Apr 2011800 DEGARELIX REDUCES URODYNAMIC CHANGES IN A RAT MODEL FOR EXPERIMENTAL DETRUSOR OVERACTIVITY AND PRODUCES MORE EFFICIENT VOIDING Giorgio Gandaglia, Fabio Benigni, Giovanni La Croce, Marco Moschini, Alice Bergamini, Frank Strittmatter, Enrico Colli, Patrizio Rigatti, Francesco Montorsi, and Petter Hedlund Giorgio GandagliaGiorgio Gandaglia Milan, Italy More articles by this author , Fabio BenigniFabio Benigni Milan, Italy More articles by this author , Giovanni La CroceGiovanni La Croce Milan, Italy More articles by this author , Marco MoschiniMarco Moschini Milan, Italy More articles by this author , Alice BergaminiAlice Bergamini Milan, Italy More articles by this author , Frank StrittmatterFrank Strittmatter Munich, Germany More articles by this author , Enrico ColliEnrico Colli Copenhagen, Denmark More articles by this author , Patrizio RigattiPatrizio Rigatti Milan, Italy More articles by this author , Francesco MontorsiFrancesco Montorsi Milan, Italy More articles by this author , and Petter HedlundPetter Hedlund Milan, Italy More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.618AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES GnRH antagonists may have beneficial effects on LUTS in patients with BPH. The aim was to study effects of degarelix, a GnRH receptor antagonist, on normal bladder function and experimental detrusor overactivity (DO) in female rats. METHODS After ethical approval, female Sprague-Dawley rats were given degarelix (10μg/kg, n=13) or saline vehicle (controls; n=11) subcutaneously every second day for 5 days. The dose regime chosen was based on the pharmacological profile of degarelix. On day 6, bladder function and responses to intravesical (ives) PGE2 (50 μM) were studied with cystometry in conscious rats. Values are mean ± SEM. ANOVA was used for comparisons. RESULTS Bladder and body weights were similar in both groups. Baseline micturition interval (MI), micturition volume (MV), and bladder capacity (BC), basal pressure (BP), and maximal pressure (MP) were similar in both groups. Degarelix rats had lower residual volumes (RV) of 0.03±0.01 ml (controls 0.10±0.02 ml, p<0.05). Threshold pressure (TP) was 28.3±3.9 (controls) and 15.2±0.8 cmH2O (degarelix, p<0.05). Flow pressure (FP) was 45.9±4.1 (controls) and 31.9±1.9 cmH2O (degarelix, p<0.05). PGE2 (ives) reduced MI, MV, and BC of all rats. RV was not changed in degarelix rats (0.04±0.01 ml) but was increased in controls to 0.15 ± 0.02 ml (p<0.001) after PGE2. Ives PGE2 increased more BP, TP, and FP in controls (p<0.001) than degarelix rats. For controls, BP, TP and FP was 24.1±4.1, 39.2±5.6, and 61.9±5.7 cmH2O, and in degarelix rats 12.1±0.9, 17.7±1.3, and 37.2±2.3 cmH2O. Whereas MP increased in controls, no change was observed for degarelix rats after ives PGE2. After intravesical PGE2, the area under the pressure curve (AUC) was 38.4±6.5 and 18.6±1.7 cmH2O/sec for controls and degarelix rats (p<0.001) and the absolute increase in AUC amounted to 14.0±3.1 (controls) and 5.1± 1.1 (degarelix; p<0.01) cmH2O/sec. In controls, compliance (C) was reduced from 0.09±0.01 at baseline to 0.05±0.01 ml/cmH2O after ives PGE2. In degarelix rats, C was unaffected by ives PGE2 and amounted to 0.15±0.04 ml/cmH2O (p<0.05). CONCLUSIONS Degarelix counteracted urodynamic signs of DO in female rats without affecting maximal bladder pressures. Indicating more efficient emptying of the bladder in this model for DO, rats treated with degarelix had less residual volume and preserved compliance. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e322 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Giorgio Gandaglia Milan, Italy More articles by this author Fabio Benigni Milan, Italy More articles by this author Giovanni La Croce Milan, Italy More articles by this author Marco Moschini Milan, Italy More articles by this author Alice Bergamini Milan, Italy More articles by this author Frank Strittmatter Munich, Germany More articles by this author Enrico Colli Copenhagen, Denmark More articles by this author Patrizio Rigatti Milan, Italy More articles by this author Francesco Montorsi Milan, Italy More articles by this author Petter Hedlund Milan, Italy More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...