Abstract
This chapter deals with repair of overlapping DNA termini. The natural cohesive termini of bacteriophage DNA, such as γ and the staggered phosphodiester bond cleavages produced by various restriction endonucleases have several possible arrangements. If the arrangement is such that a protruding 5' single-stranded end and an internal 3'-hydroxyl end are formed, then the termini will serve both as primer and template for repair synthesis by a DNA polymerase. Overlapping DNA termini can be repaired using the RNA directed DNA polymerase, which is called reverse transcriptase, from RNA tumor viruses and radioactive nucleoside triphosphates. The incorporation into the termini can be quantified and the sequence analyzed from nearest neighbor data. The chapter further explains the labeling procedure.
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