Abstract
Publisher Summary One of the most important problems in analytical biochemistry is the quantitative determination of sulfhydryl (SH) groups in proteins and low-molecular-weight compounds. Interest in the state of SH groups in proteins has increased because thiol–disulfide exchange is found to be essential in many cases for protein folding and stability. Among the low-molecular-weight compounds, glutathione (GSH) is particularly important because it is present in all animal cells and blood and is responsible for the redox state of organisms. This chapter discusses the quantitative determination of SH groups in low- and high-molecular-weight compounds by electron paramagnetic resonance (EPR). This approach combines all the advantages of the Ellman's reagent (thiol–disulfide exchange reaction) and the EPR spectroscopy: high sensitivity and the possibility of using unclear, scattering, and strained (biological) media—that is, cells and tissue.
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