Abstract
Background. Reactive oxygen species (ROS) are associated with the pathogenesis of inflammatory diseases and have a direct or indirect role in tissue damage constituting oxidative stress. ROS are also involved in impairment of β-cell function during development of diabetes, which leads to genetic ablation of KATP channels, triggering up-regulation of antioxidant enzymes. Several markers of lipid peroxidation, protein oxidation and DNA damage induced by ROS can be measured. Over the last decade, isoprostanes have been considered as the best markers of lipid peroxidation. The aim of this study was to determine the presence of 8-isoprostane in healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes and to find the correlation between 8-isoprostane levels among groups and with clinical parameters like gingival index, probing depth and clinical attachment levels. Methods. Ninety subjects were selected and divided into 3 groups: healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes (n=30 each). Saliva was collected from these subjects after obtaining consent and analyzed for 8-isoprostane levels using ELISA kit. Statistical analysis was performed using Kruskal-Wallis test, Mann-Whitney U test and Spearman’s correlation coefficient (P<0.001). Results. Statistically significant difference was found in the levels of 8-isoprostane between healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes and with all clinical parameters. Conclusion. 8-isoprostane can be considered as a pathophysiological marker to measure oxidative stress in periodontal diseases.
Highlights
IntroductionOxidative stress (OS) occurs due to an imbalance between pro-oxidant/antioxidant status, resulting in the generation of reactive oxygen species (ROS) and subsequent modification of biomolecules such as proteins, lipids and nucleic acids and changes in the organism’s structure and functions.[1] Oxidative stress has a beneficial role in the physiologic adaptation and in the regulation of intracellular signal transduction
JODDD, Vol 12, No 4 Autumn 2018Oxidative stress (OS) occurs due to an imbalance between pro-oxidant/antioxidant status, resulting in the generation of reactive oxygen species (ROS) and subsequent modification of biomolecules such as proteins, lipids and nucleic acids and changes in the organism’s structure and functions.[1]
Significant difference was found in the levels of 8-isoprostane between healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes and with all clinical parameters
Summary
Oxidative stress (OS) occurs due to an imbalance between pro-oxidant/antioxidant status, resulting in the generation of reactive oxygen species (ROS) and subsequent modification of biomolecules such as proteins, lipids and nucleic acids and changes in the organism’s structure and functions.[1] Oxidative stress has a beneficial role in the physiologic adaptation and in the regulation of intracellular signal transduction. Various studies have emphasized the association between periodontal disease and oxidative stress in recent years.[4,5] There are several mechanisms involved in the genesis of oxidative stress in diabetic patients. Reactive oxygen species (ROS) are associated with the pathogenesis of inflammatory diseases and have a direct or indirect role in tissue damage constituting oxidative stress. Several markers of lipid peroxidation, protein oxidation and DNA damage induced by ROS can be measured. The aim of this study was to determine the presence of 8-isoprostane in healthy, chronic periodontitis and chronic periodontitis subjects with type II diabetes and to find the correlation between 8-isoprostane levels among groups and with clinical parameters like gingival index, probing depth and clinical attachment levels
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Dental Research, Dental Clinics, Dental Prospects
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
