8-Hydroxy-2’-deoxyguanosine as a biomarker of oxidative stress in acute exacerbation of chronic obstructive pulmonary disease

Xing Liu,Yunshi Zhang,Ganzhu Feng,Xiaoqin Weng,Sixia Chen,Kaili Deng,Yang Zhang,Jing Yao,Tianming Gao

doi:10.3906/sag-1807-106

Abstract

8-Hydroxy-2’-deoxyguanosine (8-OHdG) is a biomarker of oxidative stress and has been implicated in many diseases. The aim of this study was to investigate the clinical value of plasma 8-OHdG level in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). A total of 154 subjects were enrolled in this study, including 20 healthy volunteers, 24 COPD patients in the stable phase, and 110 AECOPD patients. Peripheral blood samples, demographic information, and clinical characteristics were collected from all subjects at the time of being recruited into the study. Plasma 8-OHdG level was detected by enzyme-linked immunosorbent a ss ay. 8-OHdG was increased in patients with AECOPD compared to healthy subjects and patients with stable COPD, especially in smokers. It also increased with the GOLD stage, mMRC grade, CAT score, and group level of combined COPD assessment. Additionally, further analysis revealed that 8-OHdG was negatively correlated with FEV1, FEV1% predicted, and FEV1/FVC and positively correlated with C-reactive protein, procalcitonin, and neutrophil CD64. 8-OHdG is associated with spirometric severity, symptomatic severity, exacerbation risk, and inflammatory biomarkers in AECOPD patients, suggesting it as a promising biomarker for reflecting disease severity and guiding the choice of optimal therapeutic decision.

Highlights

Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation and this pathologic change is poorly reversible
The diagnosis and severity assessment of COPD were based on the Global Initiatives for Chronic Obstructive Lung Disease (GOLD) guidelines, and acute exacerbation was defined as an acute worsening condition from stable state, which presented with aggravated dyspnea, increased sputum volume, and/or changed sputum color, or any combination of these symptoms and requirement to alter the treatment plan [5]
We found that plasma 8-OHdG levels were significantly higher in acute exacerbation of chronic obstructive pulmonary disease (AECOPD) patients with GOLD stage 3 and 4 than those with GOLD stage 1 (0.48 ± 0.18 vs. 0.35 ± 0.19 ng/mL, P < 0.05; 0.58 ± 0.19 vs. 0.35 ± 0.19 ng/mL, P < 0.05, respectively) and 8-OHdG levels were higher in GOLD stage 4 patients compared with those in GOLD stage 2 (0.58 ± 0.19 vs. 0.41 ± 0.18 ng/mL, P < 0.05)

Summary

Introduction

Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation and this pathologic change is poorly reversible. The airflow limitation is associated with structural remodeling of small airways, which can be precipitated by pollutants such as toxic gases and harmful particulates [1,2]. Some patients experience relatively stable courses while others suffer frequent acute exacerbations [3,4]. The frequency of acute exacerbation accelerates the decline of lung function, which impairs the quality of life and increases health care utilization [1,5,6]. Prevention, diagnosis, and treatment of acute exacerbation are crucial for COPD patients

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