8-Hydroxy-2'-Deoxyguanosin in Peripheral Leukocyte Associated With HBsAg in Patients with Chronic Hepatitis B

Abstract

Background: Chronic hepatitis B (CHB), with accumulation of Hepatitis B surface Antigen (HBsAg) in hepatocytes, linked to the immune-mediated hepatic inflammation and induction of oxidative stress. 8-Hydroxyl-2'-deoxyguanosine (8-OHdG) is a useful biomarker for measuring the adverse effects of exogenous infectious agents in oxidative damage to DNA. Objectives: The current study aimed at investigating the possible oxidative adverse effects of HBsAg and systemic DNA damage in patients with CHB, and supporting the host-viral interaction in immune-mediated inflammatory. Methods: Thirty patients with CHB who had undergone liver biopsies for therapeutic purposes and 30 matched controls from a healthy population were randomly selected in the present study for assessment of 8-OHdG levels in peripheral blood leukocytes DNA by 32P-postlabeling analysis. Expression of HBsAg in hepatocytes was evaluated immunohistochemically in liver biopsies of patients with CHB. The effect of 8-OHdG and 95% confidence interval (CI), adjusted by relevant confounders, were assessed on hepatitis B virus (HBV) infection. Results: Experimental investigation showed increased levels of DNA adduct 8-OHdG compared with healthy individuals (mean ± standard deviation, 1456 ± 1275 vs. 402 ± 271; P < 0.001). The logistic regression with continuous and dichotomous models revealed the strong impact of 8-OHdG on CHB infection (OR = 1.20; 95%CI: 1.01 - 1.44, P = 0.043) and (OR = 7.18; 95%CI: 1.32 - 39.02, P = 0.022). HBV DNA and hepatic expression of HBsAg had a borderline association with DNA adduct 8-OHdG (r = 0.35, P = 0.054 and r = 0.36, P = 0.05). Conclusions: The current study showed that the adduct of 8-OHdG in peripheral blood cells DNA increased in patients with CHB compared with healthy carriers and the pathophysiologic role of HBsAg in oxidative stress in patients with CHB. Nonetheless, the lack of efficient DNA repair enzymes activity as well as a proper diet with antioxidant agents in CHB need to be clarified in future studies.