Abstract
Several methods based on migration of melanin granules within amphibian melanophores are currently available for the bioassay of hormones affecting pigmentation. These hormones, which include the MSH's (melanoeyte stimulating hormone) and ACTH's (adrenoeortieotropie hormone), cause the melanin granules to disperse that results in darkening of the skin and a consequent decrease in reflection of incident light by the darkened skin. This decrease in reflection of incident light by the darkened skin forms the basis for the bioassay of pigmentary hormones as developed by Shizume et al. utilizing the isolated skin of the frog. This method is designed so that the reference standard and unknown are compared under identical conditions, the biologic response and its variation are objectively quantitated, and finally, the plan of the assay permits the use of dose-response lines.
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