791 CHRONIC HYPERLIPIDEMIA CAUSES DETRUSOR OVERACTIVITY AND INCREASE IN URINARY PROSTAGLANDIN E2 RELEASE IN RABBITS

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You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research1 Apr 2011791 CHRONIC HYPERLIPIDEMIA CAUSES DETRUSOR OVERACTIVITY AND INCREASE IN URINARY PROSTAGLANDIN E2 RELEASE IN RABBITS Masaki Yoshida, Koichi Masunaga, Takashi Nagata, Akito Inadome, Yutaka Miyamoto, Tomomi Haba, and Junzo Kudoh Masaki YoshidaMasaki Yoshida Kumamoto, Japan More articles by this author , Koichi MasunagaKoichi Masunaga Tokyo, Japan More articles by this author , Takashi NagataTakashi Nagata Saitama, Japan More articles by this author , Akito InadomeAkito Inadome Kumamoto, Japan More articles by this author , Yutaka MiyamotoYutaka Miyamoto Kumamoto, Japan More articles by this author , Tomomi HabaTomomi Haba Kumamoto, Japan More articles by this author , and Junzo KudohJunzo Kudoh Kumamoto, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.609AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Recently, we demonstrated that chronic hyperlipidemia caused bladder dysfunction including detrusor overactivity in heritable hyperlipidemic rabbits (WHHLMI rabbits). Various pathologic conditions cause prostaglandins synthesis in both bladder smooth muscle and mucosa, resulting in bladder dysfunction. To evaluate the effects of hyperlipidemia on lower urinary tract function, we examined the relationship between hyperlipidemia-induced bladder dysfunction and urinary prostaglandin E2 (PGE2) level in WHHLMI rabbits. METHODS WHHLMI rabbits and the age and sex-matched Japanese white rabbits (control group) were prepared. In all rabbits, the urinary volume and frequency were recorded for three days, and 24-h urine samples of all rabbits were collected. Cystometrograms were performed under anesthesia using constant infusion of saline into the bladder to elicit voiding, and voided volume, residual urine, micturition pressure, and micturition interval were evaluated. 24-h urinary PGE2 levels were assayed by enzyme immunoassay (EIA) by using PGE2 EIA kit-Monoclonal. In addition, immunohistochemical staining of COX-2 of bladder tissue of WHHLMI and control rabbits was performed, using mouse monoclonal anti-COX-2 antibody. RESULTS The number of micturition per day was higher in WHHLMI rabbits (4.24±1.07 times/day) than in control rabbits (1.8±0.3 times/day), and the voided volume was lower in WHHLMI rabbits (14.81±4.60 ml) than in control rabbits (43.8±5.1 ml). In cystometrograms, WHHLMI rabbits showed detrusor overactivity, higher frequency of micturition (7.55±1.25 times/20 min) and lower voided volume (6.92±1.5 ml) than in control rabbits (1.44 ± 2.51 times/20 min and 20.8±3.2 ml, respectively). The urinary excretion of PGE2 was significantly higher in WHHLMI rabbits (6.44±1.87 ng/ml) than in control rabbits (0.91±0.14 ng/ml). Analysis of relationship between parameters of bladder function and urinary PGE2 level in WHHLMI rabbits indicated a significantly negative correlation (r=0.81) between urinary PGE2 level and voided volume, and a significant positive correlation between urinary PGE2 level and the number of micturition (r=0.86). Immunohistochemical staining of COX-2 showed a significant higher immunoreactivity in bladder mucosa of WHHLMI rabbits, as compared to control. CONCLUSIONS The present study suggested that chronic hyperlipidemia caused increase of COX-2 activity in bladder mucosa, which might cause the enhanced PGE2 release, resulting in activation of sensory input and detrusor overactivity. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e318 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Masaki Yoshida Kumamoto, Japan More articles by this author Koichi Masunaga Tokyo, Japan More articles by this author Takashi Nagata Saitama, Japan More articles by this author Akito Inadome Kumamoto, Japan More articles by this author Yutaka Miyamoto Kumamoto, Japan More articles by this author Tomomi Haba Kumamoto, Japan More articles by this author Junzo Kudoh Kumamoto, Japan More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...