Abstract
Reorganization of fibrin is the first stage in the matrix maturation process of bioprinted skin and takes place over a few hours to a few days. Fibrin is a popular component of many bioinks and a provisional component of the extracellular matrix present during wound healing. Cells embedded in fibrin gels attach and exert traction forces to realign and/or degrade the fibrin fibers, and sense and respond to the surrounding substrate in a mechanical feedback loop. Such cellular actions may remodel the printed construct in such a way, rendering it no longer feasible for implantation.
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