Abstract
Studies were conducted to determine if near infrared reflectance spectrophotometry (NIR) could be used to rapidly determine nonstructural carbohydrate (TNC) concentrations in marigold tissue. Marigold seedlings were grown in natural light growth chambers. Light intensity and CO2 concentration were adjusted to establish leaf samples with a wide range of the carbohydrate concentration. NIR spectra were collected on dry, ground samples using a reflectance scanning monochromator. Conventional laboratory analysis values for the same samples were correlated with spectral data. Calibration statistics were as follows: TNC; standard error of calibration (SEC)= 1.73, R2= 0.95, standard error of prediction or perfomance (SEP) = 1.64, R2= 0.95; Starch, SEC = 1.35, R2= 0.96, SEP = 1.37, R2= 0.94; Soluble Sugars, SEC = 0.26, R2= 0.91, SEP = 0.29, R2= 0.84. These results show that the NIR method can be used to rapidly determine concentrations of nonstructural carbohydrates in marigold leaf tissue and suggest the possibility for routine assessment of carbohydrate status for optimum management of environmental factors.
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