Abstract
Long term exposure to high benzyladenine (BA) concentrations inhibited shoot elongation and root growth of Cymbidium cv. Yuh Hwa rhizome explants. Through transfer experiments, it was determined that the commitment to shoot induction occurred between 10 and 14 days of exposure to at least 2.5 μM BA. BA when supplied at 20 μM during the fit 14 days of culture was found to be sufficient to induce shoot formation. Both shoot elongation and root formation were greatly improved by decreasing the BA concentration. By using radiolabelled BA, adenine was found to be a major metabolite in the rhizome tissue. Free IAA levels, quantitated by GC-MS, did not correlate well with the organogenesis of rhizomes, nor did the levels of free IAA correlate well with the activities of peroxidase and IAA oxidase, indicating a complex relationship between hormone concentration and differentiation.
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